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Sanger sequencing: Common primers provided by the Nevada Genomics Center
All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...
unr.edu5y
Sanger sequencing: Optimal amount of template and primer
A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...
Nature3y
Scientists Can Make Copies of a Gene through PCR
During these thermal cycles, DNA primers bind to the target DNA sequence, enabling DNA polymerases to assemble copies of the target sequence in large quantities. PCR makes it possible to produce ...
Kaleido Scope4y
Sample Preparation and Management
All samples and primers must be dissolved in distilled water and must be provided at the concentrations below. For each sequencing reaction submit 10 μL of template and 10 μL of primer (templates and ...
Nature3y
DNA Sequencing Technologies Key to the Human Genome Project
The inserts were sequenced using primers matching the vector sequence flanking the genomic DNA insert, and overlapping shotgun clones were used to generate a DNA sequence spanning the entire BAC ...
Kaleido Scope1y
Genomics Core Laboratories
The Genomics Core now has an Illumina NextSeq500 Next Generation Sequencing (NGS) instrument! This will expand our capacity of services already offered for NGS. Feel free to call or email us to find ...